Search results for " REEs"
showing 10 items of 18 documents
Bryophyte Species Richness on Retention Aspens Recovers in Time but Community Structure Does Not
2014
Green-tree retention is a forest management method in which some living trees are left on a logged area. The aim is to offer ‘lifeboats’ to support species immediately after logging and to provide microhabitats during and after forest re-establishment. Several studies have shown immediate decline in bryophyte diversity after retention logging and thus questioned the effectiveness of this method, but longer term studies are lacking. Here we studied the epiphytic bryophytes on European aspen (Populus tremula L.) retention trees along a 30-year chronosequence. We compared the bryophyte flora of 102 ‘retention aspens’ on 14 differently aged retention sites with 102 ‘conservation aspens’ on 14 d…
Cellobiose fermentation by Saccharomyces cerevisiae: Comparative analysis of intra versus extracellular sugar hydrolysis
2018
Abstract A prevalent procedure for the production of second generation bioethanol makes use of engineered yeast strains capable to hydrolyze cellobiose either in the cytosol or extracellularly. These two approaches have been compared in this study. For intracellular cellobiose hydrolysis, we initially tested three recombinant Saccharomyces cerevisiae strains that produced different cytosolic β-glucosidases and the cellodextrin transporter Po_CdtC from Penicillium oxalicum . The strain coexpressing Po_CdtC and the β-glucosidase from Neurospora crassa (NcBgl) showed the highest cellobiase activity but its growth in cellobiose was limited by sugar intake. A search of alternative cellobiose per…
Evolución y distribución sectorial de las fusiones de empresas españolas
2000
Es evidente que los procedimientos de fusión han sido empleados por las empresas españolas como estrategias para afrontar los retos competitivos en sus diferentes entornos económicos y, aunque han sido estudiados sus causas y resultados; el impacto global en el contexto de la economía española y su utilización real no han sido suficientemente investigados, sobre todo, debido a la falta de bases estadísticas apropiadas. En este trabajo, partiendo de la información de las últimas dos décadas, se analiza cuál ha sido su empleo efectivo, características y evolución, así como aquellos sectores más intensamente afectados. Para obtener una base que permita vislumbrar las posibles tendencias futura…
De los cruces entre el cine y la literatura: Osvaldo Soriano y la desmitificación del Olimpo hollywoodense
2014
Resumen : Este articulo se propone investigar la recuperacion del discurso cinematografico operada por el escritor argentino Osvaldo Soriano. El autor retoma personajes arquetipicos, esquemas narrativos y convenciones descriptivas de los principales generos cinematograficos hollywoodenses y las adapta al contexto cultural argentino. En particular se analizara la relacion que se establece entre la novela Cuarteles de invierno y el genero western. Se trata de una rescritura parodica en la que Soriano, desmitificando los valores tradicionalmente asociados al genero, transmite un punto de vista critico sobre el presente historico. Palabras Clave :Soriano, desmitificacion, cine, western, reescri…
Género, raza y trabajo en los almacenes de Amazon en Estados Unidos
2021
Analysis of geochemical tracers in different systems soil-Citrus limon (L.) Osbeck
Today is increasing the attention of consumers for the origin of food and high reputation of products with a distinct geographical identity. Food traceability is an important issue in food safety and quality control, with impacts on food security, its quantity and overall availability. The knowledge of a chemistry relationship between the soil and the agricultural products is an important tool for the quality assessment of food. Citrus Limon is the most important fruit tree crop in the world and the detection of potential fraud could improve by using tools linking the chemistry composition of this production to its typical growing area. This study use rare earth elements (REEs) as geochemic…
Expression and Secretion of Barley Cysteine Endopeptidase B and Cellobiohydrolase I in Trichoderma reesei.
2006
Localization of expression and secretion of a heterologous barley cysteine endopeptidase (EPB) and the homologous main cellobiohydrolase I (CBHI) in a Trichoderma reesei transformant expressing both proteins were studied. The transformant was grown on solid medium with Avicel cellulose and lactose to induce the cbh1 promoter for the synthesis of the native CBHI and the recombinant barley protein linked to a cbh1 expression cassette. Differences in localization of expression between the two proteins were clearly indicated by in situ hybridization, indirect immunofluorescence, and immunoelectron microscopy. In young hyphae, native-size recombinant epb mRNA was localized to apical compartments…
Maturation of barley cysteine endopeptidase expressed in Trichoderma reesei is distorted by incomplete processing
2002
Maturation of barley cysteine endopeptidase B (EPB) in Trichoderma reesei was studied with metabolic inhibitors, Western blotting, and immuno microscopy. The inactive 42-kDa recombinant EPB proprotein, first detected in apical cells, was sequentially processed in a time-dependent manner to a secreted polypeptide of 38.5 kDa, and thereafter, to polypeptides of 37.5, 35.5, and 32 kDa exhibiting enzyme activity both in the hyphae and culture medium. The sizes of the different forms of recombinant EPB were in accordance with molecular masses calculated from the deduced amino acid sequence, assuming cleavage at four putative Kex2p sites present in the 42-kDa proprotein. Both the liquid and the z…
Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina
2006
The mould Hypocrea jecorina (Trichoderma reesei) has two genes coding for enzymes with high similarity to the NADP-dependent glycerol dehydrogenase. These genes, called gld1 and gld2, were cloned and expressed in a heterologous host. The encoded proteins were purified and their kinetic properties characterized. GLD1 catalyses the conversion of d-glyceraldehyde and l-glyceraldehyde to glycerol, whereas GLD2 catalyses the conversion of dihydroxyacetone to glycerol. Both enzymes are specific for NADPH as a cofactor. The properties of GLD2 are similar to those of the previously described NADP-dependent glycerol-2- dehydrogenases (EC 1.1.1.156) purified from different mould species. It is a reve…
Valorization of Citrus spp. Cultivars cultivated in Sicily for the industrial use of citrus derivatives and in particular for potential alternative u…
2021
[ES] En esta tesis doctoral se discuten una serie de cuestiones científicas relacionadas con a la producción y el procesamiento de algunas especies de cítricos de especial relevancia en Sicilia y en el área mediterránea. Los objetivos se centran en estudiar la trazabilidad y sostenibilidad de la producción de algunas especies de cítricos, así como encontrar nuevas aplicaciones a los subproductos y productos de desecho de la industria, con el fin de mejorar su rentabilidad. Por lo tanto, los temas que se desarrollarán serán los siguientes: caracterización de los compuestos bioactivos presentes en aceites esenciales y en el hidrolato de diferentes especies de cítricos; seguimiento del efecto …